Senior Associate Scientist Lung Cancer Initiative

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At J&J, we are working to build a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease encourages us. We bring together the best minds and pursue the most promising science. We collaborate with the world for the health of everyone in it. The Lung Cancer Initiative (LCI), which is a cross-sector initiative, is charged by the Executive Committee of J&J with relevantly altering the course of lung cancer and improving survival by preventing, intercepting, and curing lung cancer. We are seeking for highly motivated Associate Scientist to complement our Discovery Biology team at Beerse, Belgium. The successful candidate will join a dynamic, multi-disciplinary team of scientists in exploratory immuno-oncology, playing a key role in the evaluation and understanding of new drug concepts in vitro and in vivo. Your key responsibilities: Conduct in vitro human and ex vivo mouse immunology experiments to support ongoing immune-oncology portfolio including assays using cancer cell, myeloid and T-cell based co-cultures. Tumour cell biology analysis including analysis of PDX lines and tumour dissociated material. Design and conduct in vivo syngeneic tumour model studies, to analyse efficacy of both intra-tumoral and systemic interventions. Coordinate in vivo and IHC activities from different programs. Maintain detailed and organized experimental records and stay compliant on all relevant internal and external training requirements. Application of diverse expertise in cellular and molecular biology (e.g. cell/organoid culture, compound screening, FACS, ELISA, western blotting, qRT-PCR, cell transfections & transductions, viral productions). Support discovery biology team with experimental set-up, assay optimization and data interpretation. Qualifications Required Qualifications A minimum of a BS degree with a minimum of 5 to 8 years’ laboratory-based experience in industry/biotech or an academic setting is required. Highly experienced with animal handling, subcutaneous and orthotopic cancer cell implantation (mammary, pancreas, liver, kidney, brain and colon). Drug administration by subcutaneous, intraperitoneal, intravenous tail vein injections and oral gavage. Blood collection by tail vein, submandibular vein, retro-orbital vein and cardiac puncture. Perform animal autopsy/dissection and microsurgery, perfusion and tissue collection Significant practical experience of running murine syngeneic tumour models and ex vivo immunological analysis of the tumour microenvironment is required. Experience with cell culture (hematopoietic stem cells, primary immune cells, cancer cells). Experience with immune cell isolation is required Experience and knowledge with histological analysis of tissue (tumour) samples (fixation, embebbing, sectioning and staining) Immunohistochemistry and immunocytochemistry, microscopical imaging and analysis is highly recommended. Proven experience with molecular biology techniques ; RNA/DNA/protein extraction, PCR/RT-PCR/qPCR, Western blot, molecular cloning, oligo design, immunoprecipitation and DNA manipulations. Plasmid design, purification, screening and quantification. Experience with programming & operating pipetting robots, setting up high HTS screening platforms is highly desirable FELASA B or C certificate is required Learning agility and ability to work across projects highly desirable Excellent written and verbal communication, and ability to present scientific content Excellent knowledge of Microsoft Office and Graphpad Prism. We are an equal opportunity employer and value diversity at our company. We do not discriminate on the basis of race, religion, color, national origin, gender, sexual orientation, age, marital status, veteran status, or disability status. Ready to be part of something great? Don’t forget to apply. We want YOU! Primary Location Belgium-Antwerp-Beerse- Organization Janssen Pharmaceutica N.V. (7555) Job Function R&D Requisition ID 2105979038W